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  • ActiPix™ Publications 02.03.15 (pdf, 213kb)

    Publications using the ActiPix™ D100 to date 02.03.15

  • Physicochemical characterization of a PEGylated liposomal drug formulation using capillary electroph (pdf, 0.84MB)

    In this work, the applicability of using CE to perform a physicochemical characterization of a PEGylated liposomal drug formulation of the anti-cancer agent oxaliplatin was examined. Characterization of the liposomal drug formulation using CE instrumentation encompassed: determination of the electrophoretic mobilities, size determination by Taylor dispersion analysis and interaction studies.

  • Malvern Instruments and Paraytec enter into development and technology licensing agreement (pdf, 87.67kb)

    20 July 2011; Malvern, UK: Malvern Instruments Ltd (Malvern, UK) and Paraytec Ltd (York, UK) have entered into a development and licensing agreement that will see Paraytec’s ActiPix™® technology being added to Malvern’s materials characterization portfolio. The agreement will give Malvern exclusive rights to further develop, market and sell ActiPix™® UV area imaging technology in specific fields of use related to the characterization of complex fluids for applications in biopharmaceuticals, foods and personal care industrial sectors.

  • New paper from DeMontfort University using Actipix technology (pdf, 0.79MB)

    The influence of the surfactants of sodium lauryl sulfate (SLS) and Tween 80 on carbamazepine–nicotinamide (CBZ–NIC) cocrystal solubility and dissolution behaviour has been studied in this work.

  • American Laboratory Acticle (pdf, 2.32MB)

    Pharmaceutical Dissolution and UV Imaging, American Laboratory February 2011, Stephen Wren and Jim Lenke The performance of solid pharmaceutical products is normally assessed using the pharmacopeial in vitro dissolution test. The dissolution test is used both to ensure quality and consistency of investigational clinical and registered commercial materials, and as a tool to assist in the development of new products.

  • Insights into the Early Dissolution Events of Amlodipine Using UV (pdf, 3.06MB)

    Insights into the Early Dissolution Events of Amlodipine Using UV Imaging and Raman Spectroscopy Traditional dissolution testing determines drug release to the bulk, but does not enable an understanding of the events happening close to the surface of a solid or a tablet. UV imaging is a new imaging approach that can be used to study the dissolution behavior of chemical compounds. The UV imaging instrumentation offers recording of absorbance maps with a high spatial and temporal resolution which facilitates the abundant collection of information regarding the evolving solution concentrations. In this study, UV imaging was used to visualize the dissolution behavior of amlodipine besylate(amorphous and dihydrate forms) and amlodipine free base. The dissolution of amlodipine besylate was faster from the amorphous form than from the crystalline forms. The UV imaging investigations suggested that a solvent mediated phase transformation occurred for the amorphous amlodipine besylate and the amlodipine free base samples. Raman spectroscopy was used to confirm and probe the changes at the solid surface occurring upon contact with the dissolution media and verified the recrystallization of the amorphous form to the monohydrate. The combination of UV imaging and Raman spectroscopy is an efficient tool to obtain a deeper insight into the early events of the dissolution process.

  • Monitoring Lidocaine Single-Crystal Dissolution by Ultraviolet Imaging (pdf, 344kb)

    Published online in Wiley Online Library ( DOI 10.1002/jps.22532 ABSTRACT: Dissolution critically affects the bioavailability of Biopharmaceutics Classification System class 2 compounds. When unexpected dissolution behaviour occurs, detailed studies using high information content technologies are warranted. In the present study, an evaluation of real-time ultraviolet (UV) imaging for conducting single-crystal dissolution studies was performed. Using lidocaine as a model compound, the aim was to develop a setup capable of monitoring and quantifying the dissolution of lidocaine into a phosphate buffer, pH 7.4, under stagnant conditions. A single crystal of lidocaine was placed in the quartz dissolution cell and UV imaging was performed at 254 nm. Spatially and temporally resolved mapping of lidocaine concentration during the dissolution process was achieved from the recorded images. UV imaging facilitated the monitoring of lidocaine concentrations in the dissolution media adjacent to the single crystals. The concentration maps revealed the effects of natural convection due to density gradients on the dissolution process of lidocaine. UV imaging has great potential for in vitro drug dissolution testing. © 2011 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci

  • Focusing and Mobilization of Bacteria in Capillary Electrophoresis (pdf, 1.1MB)

    An isotachophoretic method has been developed for mobilizing and focusing bacteria. This allows quantification and detection of bacteria in a narrow zone. Very good linearity was obtained for Micrococcus lysodeikticus (also called Micrococcus luteus, studied as a model of Gramţ bacteria) in the range of 0.4  108 cells/mL to 2.9  108 cells/mL, with correlation coefficients for peak height and peak area as a function of cell concentration of 0.999 and 0.998, respectively. This method is usable on both bare and hydroxypropyl cellulose-coated fused silica capillaries. The best results were obtained using 13.6 mM Tris, 150 mM boric acid as terminating electrolyte, and 4.5 mM Tris, 50 mM boric acid, and 3.31 mM HCl as leading electrolyte. With a 33.5 cm 100 μm i.d. capillary, short migration times were obtained while maintaining very low electrical current in order to minimize any Joule heating and lysis of the bacteria. AUV area imaging detector (ActiPix™ D100, Paraytec) was used with a 109 cm100 μmi.d. capillary having three loops and four detection windows to monitor the migration behavior of M. luteus and to show the stability of the zone of the focused bacteria along the capillary. Similar results were obtained for Erwinia carotovora (a model of Gram- bacteria), and for Enterobacter cloacae and Vibrio splendidus.

  • Separation, size and charge determination of small molecules using CE in combination with UV area im (doc, 10.13kb)

    This article shows how a capillary electrophoresis instrument may be used in conjunction with UV area imaging to determine mobility, diffusion coefficient and charge of components in a mixture of small molecules. Downloadable from Chromatography Today with the link below

  • Simultaneous Evaluation of Ligand Binding Properties and Protein Size by Electrophoresis and Taylor (pdf, 258kb)

    The interplay between biophysical characteristics such as protein size and shape and protein function is difficult to ascertain using simple methods. Here, we present an approach for characterizing both protein-ligand binding as well as protein hydrodynamic radius in one operation combining electrophoresis and size measurement by dispersion using capillaries. The methodology is based on the integration of Taylor dispersion analysis and capillary electrophoresis and is here demonstrated using commercially available capillary electrophoresis instrumentation modified with a pixel sensor UV area imager,allowing two detection points along the capillary. Analytes are the human serum proteins r1-acid glycoprotein and albumin interacting with the drug propranolol in a frontal analysis mode. Upon introduction of the propranolol-protein sample, voltage is initially applied to facilitate electrophoretically mediated separation of ligand and protein and frontal analysis. Then a pressure mobilization step is used whereby Taylor dispersion can be characterized online based on the signal from the UV area imager. Estimates of ligand binding and values for hydrodynamic radii agree with values obtained by independent methods.

  • Real-Time UV Imaging of Nicotine Release from Transdermal Patch (pdf, 0.87MB)

    ABSTRACT Purpose This study was conducted to characterize UV imaging as a platform for performing in vitro release studies using Nicorette® nicotine patches as a model drug delivery system.

  • A novel approach to measurement of hydrodynamic radius for a standard protein using UV area imaging (pdf, 5.43MB)

    An early article from the June 2008 edition of Chromatography Today discussing the principles behind Taylor dispersion analysis.

  • Lab-on-Capillary: A Versatile Format for Nanolitre Scale Chemistry and Biochemistry (pdf, 1.95MB)

    Chemistry Focus article in International Laboratory, February 2007 discussing how separations in capillaries can be readily integrated with other in-capillary processes such as mixing and reaction, laying the foundations for a versatile and widely applicable lab-on-capillary format

  • CMOS Technology for Scientific Imaging (pdf, 10.71kb)

    Details of Publication: Ken Kaufman, Spectroscopy 25(7), July 2010, Pages 20-25 Summary: A review of CCDs and CMOS imaging sensors and cameras for scientific imaging. This paper will help the reader understand how CMOS detectors work which are applied in ActiPix™ products The document has details of online version of article which can be downloaded.

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