Posters & Presentations

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  • Integrated UV-Vis Imaging, Light Microscopy and Raman Spectroscopy for Characterizing Piroxicam Supe (pdf, 0.74MB)

    The aim of this study was to develop an integrated analytical flow-through setup for characterizing drug supersaturation, precipitation and dissolution using piroxicam as model compound.

  • MSB2013 Poster (pdf, 436kb)

    Demonstrate application of ActiPix™ D100 UV area imaging through a silica-based column bed

  • Surface Dissolution Imaging Symposium Presentations 2012 (zip, 38.15MB)

    The collected presentations from the 2012 Symposium in pdf format

  • Simultaneous characterization of ligand binding properties (pdf, 449kb)

    Characterization of proteins and their interactions is fundamental to the understanding of biochemical processes since most biomolecular processes involve the molecular recognition and ligand binding by proteins. Protein based drugs represent special challenges in terms of drug development and manufacturing. The structural integrity is sensitive to the conditions encountered during formulation, production and storage. The therapeutic efficacy is closely related to the conformational structure; thus development of protein drugs requires a detailed knowledge of the physical and chemical stability. In this context, it would be beneficial if biophysical and functional characterization could be combined into one method.

  • Surface Dissolution Imaging - an Introduction (pdf, 0.74MB)

    In this poster, Paraytec introduce the principles, operation and key benefits of using surface dissolution imaging to the formulations community for rapid drug development. Model compounds are used to demonstrate practicle application of this revolutionary technique.

  • The development and application of ultraviolet area imaging detection (pdf, 371kb)

    Pharmacopoeial dissolution methods are the routine test of in-vitro product performance, but whilst they measure product consistency they provide little detailed information about dissolution mechanism. Drug product dissolution is complex, and involves several more fundamental processes such as: hydration, disintegration, erosion, particle de-agglomeration, and dissolution of the drug substance. Thus what is called drug product “dissolution” is actually a combination of several processes. In this poster we present work on the development and application of UV area imaging as a tool to investigate the dissolution of the drug substance. Theories covering the dissolution of chemical substances (e.g. starting from Noyes & Whitney) stress the importance of the concentration gradient between the solid/solution interface and the bulk solution. UV imaging at the micron scale allows this concentration gradient to be investigated.

  • Novel instrumentation to gain insight into the aggregation state of proteins by Taylor Dispersion An (pdf, 0.57MB)

    The ActiPix™ D100 is a novel analytical instrument using UV area imaging and Taylor dispersion analysis (TDA)1,2 for determining diffusion coefficients and hydrodynamic radii of proteins in solution. The detector monitors broadening of a band of a therapeutic protein or small molecule solution injected into a stream of buffer solution and driven by a syringe pump through a fused-silica capillary. The band is imaged at two windows, the first on entry to and the second on exit from a loop in the capillary. The hydrodynamic radius follows from the measured differences between peak times (first moments) and variances (second moments) at the two windows3. Preliminary experimental work has focused on comparison of this technique with that of dynamic light scattering (DLS) which is routinely used for particle size characterization in solution. Preliminary results indicate good correlation between the two techniques for measuring protein (BSA, Ovalbumin, Lysozyme and IgG) and small molecule (caffeine) hydrodynamic radii. Initial Actipix measurements for protein solutions show hydrodynamic radii results in good correlation with literature values. Repeatability and precision were also tested using model protein and small molecule solutions. The ability to determine protein aggregate levels is being explored.

  • AAPS Posters 2012 (zip, 7.19MB)

    AAPS Posters 2012 Paraytec and SDI300 user posters

  • Presentation from DDA CRS Australia (pdf, 0.68MB)

    A Short presentation detailing the theoretical aspects of the SDI300 system

  • FOCUSING AND MOBILIZATION OF BACTERIA BY CAPILLARY ELECTROPHORESIS (pdf, 278kb)

  • UV Imaging Symposium Presentations 2011 (zip, 21.37MB)

    The collected presentations from the UV Imaging Symposium 2011 in pdf format

  • AAPS 2011 Posters (zip, 8.5MB)

    AAPS 2011 Posters from Paraytec Copenhagan University and Bradford University, on the ActiPix™ SDI300

  • MATCHING THEORY AND OBSERVATIONS FOR SURFACE DISSOLUTION IMAGING EXPERIMENTS (pdf, 484kb)

    Under steady state flow conditions of the dissolution medium, the key variable in determining the dissolution rate is shown to be the solubility of the API as formulated (in these examples, the neutral species), not the equilibrium solubility in the buffer.

  • UV Imaging poster HPLC2015 (pdf, 479kb)

    Imaging UV detection across 100 micron i.d. nanoLC capillary columns with single-particle frits & chromatography of intact proteins show Narrow bands immediately post-column: best signal only within first 1 mm Distance dependence readily visualised and quantified Advantage of sensitivity increase with new generation D200 detector & high frame rate Sensitivity: < 10 fmol for proteins Highly compatible with compact nanoflow pump / injector Advantage in working with high performance nano columns: no column − transfer line − detector connectors! Excellent potential for nanoLC of proteins, & rapid method development for bioseparations

  • A Prototype Immobilised Enzyme Microreactor for the Quantification of Multi-Step Enzyme Kinetics (pdf, 1.93MB)

    The Bioconversion-Chemistry-Engineering Interface Programme (BiCE) is a multidisciplinary collaboration between three academic Departments at UCL focusing on the integration of biocatalysis and chemistry with engineering. One of the outputs of the BiCE programme are families of evolved enzymes with improved conversion rates and substrate ranges [1]. In parallel we are looking at automated techniques for the high-throughput analysis of these enzymes under process conditions in particular in microwell [2] and microfluidic [3] formats.

  • Applications of CE using a looped capillary and the ActiPix™ D100 UV area imaging detector: se (pdf, 0.67MB)

    Use of the ActiPix™ UV area imaging detector with multiple windows on a single looped capillary provides new insights with species of pharmaceutical & biopharmaceutical interest. Two key applications are demonstrated: Determination of hydrodynamic radius from imaging pressure driven flow of analyte bands at two windows. Results are shown for proteins and small molecules injected as single species and in mixtures, and for a mixture of proteins with simultaneous electrophoretic separation. Determining substrate specificity of a biocatalyst towards a mixture of UV active compounds using a continuous engagement electrophoretically mediated microanalysis (EMMA) method.

  • High Definition UV Area Imaging and Detection (pdf, 0.66MB)

    An A0 poster describing the benifits of the ActiPix™ D100 for determining hydrodynamic radius

  • A New Miniature UV Imaging Detector Based on Active Pixel Sensor Technology: Applications or Single (pdf, 0.7MB)

    Presented at PittCon 2007, Chicago.

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